課程資訊
課程名稱
乳品生物技術
BIOTECHNOLOGY IN MILK PRODUCTS 
開課學期
98-2 
授課對象
生物資源暨農學院  動物科學技術學研究所  
授課教師
陳明汝 
課號
AniSci7022 
課程識別碼
626 M1580 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期四6,7,8(13:20~16:20) 
上課地點
加工204室 
備註
總人數上限:30人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/982milkbio 
課程簡介影片
 
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課程大綱
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課程概述

The objectives of this course are to study biotechnology and its implication for the future design and production of animal industry. This course will include bioreactor engineering, identification of probiotics, microencapsulation and nanoencapsulation, as well as implication of biotechnology in dairy ingredients. In order to know the newest developments in this area, I will invite the speakers to present some of those topics. 

課程目標
Overview of Key Issues
Genetically Improved Starter Strains
1. Spontaneous genetic deletion for a milk shelf-stable yogurt
2. Transfer of genes coding for exo-cellular polysaccharides for a creamier yogurt
3. Metabolic engineering of lactic acid bacteria to produce L-alanine instead of lactate
Discrimination of probiotics
4. Phenotypic: morphology, physiology, biotyping
5. Whole cell protein analysis
6. Restriction enzyme analysis -Pulsed Field Gel Electrophoresis, REA-PFGE
7. Restriction fragment length polymorphism, RFLP
8. Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction, RAPD-PCR
9. Oligonucleotide probes for detection and identification (microarray)
Bioreactor Engineering
10. Introduction to bioreactor engineering
11. Membrane bioreactors : Enzyme processes
12. Bioreactor considerations for producing flavors and pigments
13. DNA test identifies animal species in food products
Microencapsulation and Nanoencapsulation
14. Microncapsulation processes and materials
15. Microencapsulation of probiotics
16. Biodegradable microspheres: advances in production technology.
17. nanoparticles : preparation and characterization
Implication of biotechnology in dairy ingredients
18. Production of Lactoferrin by biotechnology
19. Identification of the lantibiotic nisin Q
20. Bovine chymosin: Production by rDNA technology and application in cheese manufacture
 
課程要求
Since the readings and notes are available prior to each week's class, I will come into class expecting that you have read the assigned material. I ask you to come prepared to class ready to participate, ask questions and offer comments. You are expected to attend all classes. 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
1. Shortt, C., and J. O’Brien. 2004. Hand book of functional dairy products. CRC press.
2. Mattila, T, and M. Saarela. 2003. Functional dairy products. CRC press.
3. Nesser, J., and J. B. German. 2004. Bioprocesses and biotechnology for functional foods and Nutraceuticals.
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/25  Introduction 
第2週
3/4  Social impack  
第3週
3/11  Gene technology and milk production  
第4週
3/18  Genetically Improved Starter Strains  
第5週
3/26  Genetically Improved Starter Strains  
第6週
4/1  Genetically Improved Starter Strains  
第7週
4/8  probiotics conference 
第8週
4/15  Genetically Improved Starter Strains  
第9週
4/22  identification of probiotics 
第10週
4/29  Discrimination of probiotics  
第11週
5/6  Discrimination of probiotics  
第12週
5/13  Discrimination of probiotics  
第13週
5/20  Discrimination of probiotics  
第14週
5/27  Bioreactor Engineering  
第15週
6/3  Bioreactor Engineering  
第16週
6/10  Microencapsulation and Nanoencapsulation  
第17週
6/17  Microencapsulation and Nanoencapsulation  
第18週
6/24  Final report